Journal: Cancer Science

Article Title: Down‐regulation of pescadillo inhibits proliferation and tumorigenicity of breast cancer cells

doi: 10.1111/j.1349-7006.2009.01325.x

Figure Lengend Snippet: Overexpression of pescadillo in human primary breast cancer. (A) Western blotting analysis of pescadillo protein in normal breast epithelial cells (NBEC) and indicated breast cancer cell lines. α‐Tubulin was probed for loading control. (B) Expression of pescadillo mRNA in the NBEC and cultured breast cancer cell lines. Expression levels were normalized for GAPDH. (C) Western blotting analysis of pescadillo protein from human primary breast cancer (T) and paired tumor‐adjacent non‐cancerous breast tissues (n), with each pair taken from a same patient. (D) Real time RT‐PCR analysis of pescadillo mRNA from the same four pairs of breast cancer and adjacent non‐cancerous tissues. Error bars represent SDs calculated from three parallel experiments.

Article Snippet: IHC analysis was performed to study altered protein expression in five normal breast tissues and 92 human breast cancer tissues using rabbit anti‐pescadillo antibody (1:250; Bethyl Laboratories).

Techniques: Over Expression, Western Blot, Control, Expressing, Cell Culture, Quantitative RT-PCR

Journal: Cancer Science

Article Title: Down‐regulation of pescadillo inhibits proliferation and tumorigenicity of breast cancer cells

doi: 10.1111/j.1349-7006.2009.01325.x

Figure Lengend Snippet: Increased expression of pescadillo protein in histopathological sections of breast cancer as shown by immunohistochemical analysis. (A) Representative examples of IHC staining for pescadillo expression in normal breast tissues and breast cancer of American Joint Committee on Cancer (AJCC) stages I–IV. (a,b) Normal breast tissue (c,d) clinical stage I; (e,f) clinical stage II; (g,h) clinical stage III; and (i,j) clinical stage IV. Magnifications: ×200 (a, c, e, g, and i); ×400 (b, d, f, h, and j). (B) Average mean optical densities (MODs) of pescadillo staining in all stages of breast cancers (randomly chosen 12 cases per stage) were statistically higher than that in normal breast tissues (five cases). *Statistical significance (P < 0.05).

Article Snippet: IHC analysis was performed to study altered protein expression in five normal breast tissues and 92 human breast cancer tissues using rabbit anti‐pescadillo antibody (1:250; Bethyl Laboratories).

Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Staining

Journal: Viruses

Article Title: Recombinant Pseudorabies Virus with TK/gE Gene Deletion and Flt3L Co-Expression Enhances the Innate and Adaptive Immune Response via Activating Dendritic Cells

doi: 10.3390/v13040691

Figure Lengend Snippet: Characterization of the recombinant HNX-TK − /gE − -Flt3L. ( A ) Detection of the transcription of the Flt3L gene in HNX-TK − /gE − -Flt3L virus- and HNX-infected cells through RT-qPCR (**** < 0.001). ( B ) Detection of the Flt3L protein and viral gB protein expression through an immunofluorescence assay (IFA). Green: gB-positive cells; red: Flt3L-positive cells; blue: DAPI-stained PK-15 cell nucleus. DAPI, 4’,6-diamidino-2-phenylindole. ( C ) Western blot analysis of β-actin, gD, and Flt3L of the PK-15 cells infected with HNX-TK − /gE − -Flt3L, HNX, or DMEM as a control. ( D ) One step growth curves of HNX-TK − /gE − -Flt3L and HNX in PK-15 cells.

Article Snippet: The blots were blocked by skim milk (BD, Bioscience, Inc., Saint Louis, MO, USA), and this was followed by incubation with rabbit anti-Flt3L polyclonal antibody (Bioss, Beijing, China), mouse anti-gD monoclonal antibody (Keqian Ltd., Wuhan, China), and mouse anti-β-actin monoclonal antibody (Bioss).

Techniques: Recombinant, Infection, Quantitative RT-PCR, Expressing, Immunofluorescence, Staining, Western Blot

Journal: Oncology Research

Article Title: RBMS3 Inhibits the Proliferation and Metastasis of Breast Cancer Cells

doi: 10.3727/096504017X14871200709504

Figure Lengend Snippet: RBMS3 was lowly expressed in breast cancer tissues and cell lines. (A) RBMS3 mRNA expression was determined in breast cancer tissues. (B) Western blot analysis was used to examine RBMS3 protein expression in breast cancer tissues. * p < 0.05 compared to the adjacent normal tissues. All the experiments were repeated at least three times. (C, D) The expression of RBMS3 at both mRNA and protein levels was obviously reduced in breast cancer cell lines. * p < 0.05 compared to the NBEC cell line.

Article Snippet: Human breast cancer cell lines MCF-7, MDA-MB-231, and BT-474, as well as normal human breast epithelial cell line (NBEC), were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Western Blot